Field investigations of the nest architecture of the Amazonian bumble bee, Bombus transversalis (Olivier), revealed the presence of a new species of parasitoid wasp belonging to the microgastrine braconid genus Apanteles Foerster, reared from case-bearing tineid moth caterpillars feeding within the nests. The only other two species of Apanteles Foerster known to attack moth larvae within bumble bee colonies in the New World are here reviewed and compared taxonomically and biologically. An illustrated key to the three species recognized in this paper [Apanteles galleriae Wilkinson, A. nephoptericis (Packard) and A. nidophilus Whitfield & Cameron, n. sp.] is presented, and the latter two species are fully described and illustrated for the first time.